{"title":"ECL Western Blot Substrates","description":"\u003cp data-pm-slice=\"1 1 []\"\u003e\u003cspan\u003eTanon's high-sensitivity ECL chemiluminescent substrates, designed for reliable and efficient protein detection in Western blotting applications.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAvailable in \u003c\/span\u003e\u003cstrong\u003e\u003cspan\u003eHigh-sig ECL\u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003e and \u003c\/span\u003e\u003cstrong\u003e\u003cspan\u003eFemto-sig ECL\u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003e formulations, these non-radioactive, HRP-based detection substrates offer flexible sensitivity options, from picogram (pg) to femtogram (fg) detection levels, to suit different protein expression levels and experimental requirements.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eCompatible with X-ray film and chemiluminescence imaging systems, Tanon ECL substrates deliver sensitive protein detection with a simple two-component mixing procedure, providing a practical and cost-effective solution for routine Western blot analysis.\u003c\/span\u003e\u003c\/p\u003e","products":[{"product_id":"ecl-western-blotting-substrate-high-sig","title":"ECL Western Blotting Substrate (High-sig)","description":"\u003cp data-pm-slice=\"1 1 []\"\u003e\u003cstrong\u003e\u003cspan\u003eTanon High-sig ECL Western Blotting Substrate\u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003e is a high-sensitivity, non-radioactive chemiluminescent substrate designed for the detection of horseradish peroxidase (HRP) in Western blotting applications.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eFeaturing an enhanced luminol-based formulation, High-sig ECL enables protein detection at the \u003c\/span\u003e\u003cstrong\u003e\u003cspan\u003epicogram (pg) level\u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003e, delivering sensitive chemiluminescent signals for reliable Western blot analysis.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eCompatible with both X-ray film and chemiluminescence imaging systems, the substrate provides flexible detection options for routine protein research.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe convenient two-component system consists of Luminol\/Enhancer Solution and Peroxide Buffer, mixed at a 1:1 ratio before use. Available in \u003c\/span\u003e\u003cstrong\u003e\u003cspan\u003e100 mL and 500 mL pack sizes\u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003e, High-sig ECL offers flexibility for laboratories with different testing volumes.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0767\/3158\/0659\/files\/MOPS_Running_Buffer.pdf?v=1793599245\" title=\"MOPS Running Buffer BT8100-2002H\" rel=\"noopener\" target=\"_blank\"\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/a\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0767\/3158\/0659\/files\/Tanon_High-sig_ECL_Western_Blotting_Substrate_User_Manual.pdf?v=1793613320\" title=\"Tanon_ECL_Western_Blotting_Substrate (High-sig)\" rel=\"noopener\" target=\"_blank\"\u003eDatasheet\u003c\/a\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-pm-slice=\"1 3 []\"\u003e\u003cstrong\u003e\u003cspan\u003eKey Features\u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cul data-spread=\"false\" data-pm-slice=\"3 3 []\"\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eHigh-Sensitivity Detection:\u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003e Enables antigen detection at the picogram (pg) level.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eHRP-Based Chemiluminescence:\u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003e Non-radioactive enhanced luminol-based substrate for Western blot detection.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eFlexible Imaging Compatibility:\u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003e Suitable for X-ray film and chemiluminescence imaging systems.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eSimple Preparation:\u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003e Mix Luminol\/Enhancer Solution and Peroxide Buffer at a 1:1 ratio before use.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eEfficient Detection:\u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003e Recommended membrane incubation time of approximately 1 minute.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eFlexible Pack Sizes:\u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003e Available in 100 mL and 500 mL configurations.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eConvenient Storage:\u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003e Room Temperature: Up to 6 months; 2–8°C: Up to 24 months\u003c\/span\u003e\u003cspan\u003e\u003cbr\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ctable class=\"MsoNormalTable\" border=\"1\" cellpadding=\"0\" style=\"border: 1pt solid windowtext; width: 100.063%; height: 106.79px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 35.5966px;\"\u003e\n\u003ctd style=\"border: 1pt solid windowtext; padding: 0.75pt; width: 27.9384%; height: 35.5966px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003eCatalogue Number\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border: 1pt solid windowtext; padding: 0.75pt; width: 13.6803%; height: 35.5966px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003ePack Size\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border: 1pt solid windowtext; padding: 0.75pt; width: 23.792%; height: 35.5966px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003eKit Components\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border: 1pt solid windowtext; padding: 0.75pt; width: 30.7443%; height: 35.5966px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003eMembrane Coverage\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 35.5966px;\"\u003e\n\u003ctd style=\"border: 1pt solid windowtext; padding: 0.75pt; width: 27.9384%; height: 35.5966px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e180-501\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border: 1pt solid windowtext; padding: 0.75pt; width: 13.6803%; height: 35.5966px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e100 mL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border: 1pt solid windowtext; padding: 0.75pt; width: 23.792%; height: 35.5966px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e2 × 50 mL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border: 1pt solid windowtext; padding: 0.75pt; width: 30.7443%; height: 35.5966px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003eUp to 1,000 cm²\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 35.5966px;\"\u003e\n\u003ctd style=\"border: 1pt solid windowtext; padding: 0.75pt; width: 27.9384%; height: 35.5966px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e180-5001\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border: 1pt solid windowtext; padding: 0.75pt; width: 13.6803%; height: 35.5966px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e500 mL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border: 1pt solid windowtext; padding: 0.75pt; width: 23.792%; height: 35.5966px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e2 × 250 mL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border: 1pt solid windowtext; padding: 0.75pt; width: 30.7443%; height: 35.5966px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003eUp to 5,000 cm²\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cspan\u003e\u003cbr\u003e\u003c\/span\u003e\u003c\/p\u003e","brand":"Tanon","offers":[{"title":"100 mL (code 180-501)","offer_id":68267694063859,"sku":null,"price":0.0,"currency_code":"AUD","in_stock":true},{"title":"500 mL (code 180-5001)","offer_id":68267694096627,"sku":null,"price":0.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0767\/3158\/0659\/files\/exec-d00fe935-c6c2-4eae-83a0-2e7669989f47_51d000e2-2511-497f-a63d-650f4e22e4be.png?v=1793615305"},{"product_id":"ecl-western-blotting-substrate-femto-sig","title":"ECL Western Blotting Substrate (Femto-sig)","description":"\u003cp data-pm-slice=\"1 1 []\"\u003e\u003cstrong\u003e\u003cspan\u003eTanon Femto-sig ECL Western Blotting Substrate\u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003e is an ultra-sensitive, non-radioactive chemiluminescent substrate designed for the detection of horseradish peroxidase (HRP) in Western blotting applications.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eFeaturing an enhanced luminol-based formulation, Femto-sig ECL enables antigen detection at the \u003c\/span\u003e\u003cstrong\u003e\u003cspan\u003efemtogram (fg) level\u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003e, making it suitable for detecting low-abundance proteins and weakly expressed targets in protein research.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eCompatible with both X-ray film and chemiluminescence imaging systems, the substrate provides flexible detection options for sensitive Western blot analysis.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe convenient two-component system consists of Luminol\/Enhancer Solution and Peroxide Buffer, mixed at a 1:1 ratio immediately before use. Each 100 mL kit provides sufficient working substrate for approximately 1,000 cm² of blotting membrane.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0767\/3158\/0659\/files\/Tanon_Femto-sig_ECL_Western_Blotting_Substrate_User_Manual.pdf?v=1793615777\" title=\"Tanon_ECL_Western_Blotting_Substrate (Femto-sig)\" rel=\"noopener\" target=\"_blank\"\u003e\u003cstrong\u003eDatasheet\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp data-pm-slice=\"1 3 []\"\u003e\u003cstrong\u003e\u003cspan\u003eKey Features\u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cul data-spread=\"false\" data-pm-slice=\"3 3 []\"\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eUltra-High Sensitivity:\u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003e Enables femtogram (fg)-level antigen detection for low-abundance protein analysis.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eHRP-Based Chemiluminescence:\u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003e Non-radioactive enhanced chemiluminescent substrate for Western blot detection.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eFlexible Imaging Compatibility:\u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003e Suitable for X-ray film and chemiluminescence imaging systems.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eSimple Preparation:\u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003e Mix Luminol\/Enhancer Solution and Peroxide Buffer at a 1:1 ratio before use.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eFast Signal Development:\u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003e Recommended membrane incubation time of approximately 1 minute.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eConvenient Kit Format:\u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003e Supplied as two 50 mL bottles, providing 100 mL of working substrate.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eRefrigerated Storage:\u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003e Room Temperature: Up to 6 months; 2–8°C: Up to 24 months\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Tanon","offers":[{"title":"Default Title","offer_id":68267773591795,"sku":null,"price":0.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0767\/3158\/0659\/files\/exec-6ff748ec-0bdc-4859-a183-faf5f372e512.png?v=1793615924"}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0767\/3158\/0659\/collections\/exec-1d5ba096-c212-4fcd-8956-0dfd4bf0ed76.png?v=1793613712","url":"https:\/\/cyanbiotech.com\/collections\/precast-gels-copy.oembed","provider":"Cyan Biotech Pty Ltd","version":"1.0","type":"link"}