{"product_id":"ecl-western-blotting-substrate-femto-sig","title":"ECL Western Blotting Substrate (Femto-sig)","description":"\u003cp data-pm-slice=\"1 1 []\"\u003e\u003cstrong\u003e\u003cspan\u003eTanon Femto-sig ECL Western Blotting Substrate\u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003e is an ultra-sensitive, non-radioactive chemiluminescent substrate designed for the detection of horseradish peroxidase (HRP) in Western blotting applications.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eFeaturing an enhanced luminol-based formulation, Femto-sig ECL enables antigen detection at the \u003c\/span\u003e\u003cstrong\u003e\u003cspan\u003efemtogram (fg) level\u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003e, making it suitable for detecting low-abundance proteins and weakly expressed targets in protein research.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eCompatible with both X-ray film and chemiluminescence imaging systems, the substrate provides flexible detection options for sensitive Western blot analysis.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe convenient two-component system consists of Luminol\/Enhancer Solution and Peroxide Buffer, mixed at a 1:1 ratio immediately before use. Each 100 mL kit provides sufficient working substrate for approximately 1,000 cm² of blotting membrane.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0767\/3158\/0659\/files\/Tanon_Femto-sig_ECL_Western_Blotting_Substrate_User_Manual.pdf?v=1793615777\" title=\"Tanon_ECL_Western_Blotting_Substrate (Femto-sig)\" rel=\"noopener\" target=\"_blank\"\u003e\u003cstrong\u003eDatasheet\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp data-pm-slice=\"1 3 []\"\u003e\u003cstrong\u003e\u003cspan\u003eKey Features\u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cul data-spread=\"false\" data-pm-slice=\"3 3 []\"\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eUltra-High Sensitivity:\u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003e Enables femtogram (fg)-level antigen detection for low-abundance protein analysis.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eHRP-Based Chemiluminescence:\u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003e Non-radioactive enhanced chemiluminescent substrate for Western blot detection.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eFlexible Imaging Compatibility:\u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003e Suitable for X-ray film and chemiluminescence imaging systems.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eSimple Preparation:\u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003e Mix Luminol\/Enhancer Solution and Peroxide Buffer at a 1:1 ratio before use.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eFast Signal Development:\u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003e Recommended membrane incubation time of approximately 1 minute.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eConvenient Kit Format:\u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003e Supplied as two 50 mL bottles, providing 100 mL of working substrate.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eRefrigerated Storage:\u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003e Room Temperature: Up to 6 months; 2–8°C: Up to 24 months\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Tanon","offers":[{"title":"Default Title","offer_id":68267773591795,"sku":null,"price":0.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0767\/3158\/0659\/files\/exec-6ff748ec-0bdc-4859-a183-faf5f372e512.png?v=1793615924","url":"https:\/\/cyanbiotech.com\/products\/ecl-western-blotting-substrate-femto-sig","provider":"Cyan Biotech Pty Ltd","version":"1.0","type":"link"}