Tanon

ECL Western Blotting Substrate (Femto-sig)

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Description

Tanon Femto-sig ECL Western Blotting Substrate is an ultra-sensitive, non-radioactive chemiluminescent substrate designed for the detection of horseradish peroxidase (HRP) in Western blotting applications.

Featuring an enhanced luminol-based formulation, Femto-sig ECL enables antigen detection at the femtogram (fg) level, making it suitable for detecting low-abundance proteins and weakly expressed targets in protein research.

Compatible with both X-ray film and chemiluminescence imaging systems, the substrate provides flexible detection options for sensitive Western blot analysis.

The convenient two-component system consists of Luminol/Enhancer Solution and Peroxide Buffer, mixed at a 1:1 ratio immediately before use. Each 100 mL kit provides sufficient working substrate for approximately 1,000 cm² of blotting membrane.

Datasheet

Key Features

  • Ultra-High Sensitivity: Enables femtogram (fg)-level antigen detection for low-abundance protein analysis.

  • HRP-Based Chemiluminescence: Non-radioactive enhanced chemiluminescent substrate for Western blot detection.

  • Flexible Imaging Compatibility: Suitable for X-ray film and chemiluminescence imaging systems.

  • Simple Preparation: Mix Luminol/Enhancer Solution and Peroxide Buffer at a 1:1 ratio before use.

  • Fast Signal Development: Recommended membrane incubation time of approximately 1 minute.

  • Convenient Kit Format: Supplied as two 50 mL bottles, providing 100 mL of working substrate.

  • Refrigerated Storage: Room Temperature: Up to 6 months; 2–8°C: Up to 24 months

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